6. 植物の低温適応における脂質の役割(セミナー「ストレスと生物のレスポンス」)

概要

論文の詳細を見る
Roles of the unsaturation of membrane lipids in low-temperature adaptation were studied using strains of cyanobacteria, Synechocystis PCC6803 and Anacystis nidulans R2-SPc that were gene-technologically manipulated with a desaturase gene. In the case of Synechocystis PCC6803, the unsaturation of membrane lipids was eliminated stepwise and discretely. The wild type contained mono-, di- and triunsaturated lipid molecules; a mutant, designated Fad6, contained mono- and diunsaturated lipid molecules; and a transformant of Fad6 with a disrupted gene for desaturation, designated Fad6/desA::Km^r contained only monounsaturated lipid molecules. Fad6/desA::Km^r was the most susceptible to low temperature among these strains with respect to growth and photoinhibition, whereas Fad6 and wild type were apparently indistinguishable in terms of sensitivity to growth and photoinhibition at low temperature. The introduction of a desaturase gene from Synechocystis PCC6803 into A. nidulans R2-SPc increased the unsaturation of membrane lipids and the tolerance of the recipient to low temperature. These results suggest that the unsaturation of membrane lipids is important for the tolerance toward low temperature.
低温生物工学会の論文
1993-10-20