7. 植物寄生性糸状菌の乾燥及び凍結による保存(昭和60年度第31回凍結及び乾燥研究会研究報告,故根井外喜男先生追悼号)
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Viability of conidia or sclerotia of various fungi subjected to L-drying and freeze-drying was tested after storage for 47 to 209 days at 5℃ and 47 to 203 days at 37℃, respectively. The viability of spores of obligate parasitic fungi subjected to programmed freezing and of mycelial disks of Mastigomicotina subjected to freezing at -80℃ in a freezer or in liquid nitrogen was tested immediately after treatment of after storage for 19 to 80 days. The conidia or sclerotia subjected to L-drying showed a high viability when L2 was used as suspending medium, although the conidia of Botrytis cinerea and Pyricularia oryzae showed a low viability after L-drying. The conidia and sclerotia subjected to freeze-drying showed a lower viability than after L-drying. The conidia of Pseudoperonospora cubensis subjected to programmed freezing showed a higher viability when 10% dimethyl sulfoxide (DMSO)was used as suspending medium. The conidia of Erysiphe pisi subjected to programmed freezing showed a very high viability when 5% DMSO+5% sucrose was used as suspending medium. The homogenized suspension of club root containing resting spores of Plasmodiophora brassicae subjected to freezing at -80℃ showed a high viability regardless of suspending media used. The mycelial disks of Phytophthora spp. subjected to freezing at -80℃ showed a high viability when 5% or 10% DMSO, 5% glycerol, 5% sucrose, or 5% DMSO+10% sucrose were used as suspending media, but the disks of P. capsici did not survive when subjected to the same tests. The viability of the mycelial disks of Pythium spp. fluctuated in the same tests. The mycelial disks of Phytophthora spp. and Pythium spp. lost their viability after being subjected to freezing in liquid nitrogen.
- 低温生物工学会の論文
- 1985-09-30
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