16. ブロムグラス懸濁培養細胞培地に分泌される低温誘導性キチナーゼの特徴と不凍活性(平成20年度第54回低温生物工学会年会)

概要

論文の詳細を見る
Antifreeze activity was induced in the medium of suspension cells of bromegrass (Bromus inermis) by incubation of the cultures at 4℃. We isolated a cold-inducible chitinase gene of bromegrass (BiCHT1) whose product is highly homologous to rye CHT9 known as an antifreeze protein. BiCHT1 was over-expressed in bromegrass cells using genetic transformation procedures. BiCHT1 products had chitinase activity, but the expressed proteins did not affect the growth of ice crystals in any conditions tested. The results of transgenic analyses suggest that the BiCHT1 product is a major protein with chitinase activity secreted in the medium of cold-treated cells but is unlikely to be responsible for the antifreeze activity detected in the culture medium.
2008-12-30