1. Hydroxyl groups of threonines contribute to the activity of Ca^<2+>-depdendent type II antifreeze protein

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概要

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• Type II antifreeze protein from herring and smelt (AFPII) exhibits thermal hysteresis (TH) activity when the protein is saturated with Ca^<2+>. The ice-binding site of AFPII consists of Thr96, Thr98, and two Ca^<2+>-coordinating residues. Here we examined TH activity of AFPII from Japanese smelt (Hypomesus nipponensis) and its mutant proteins, T96S, T96V, T96A, T98S, T98V, and T98A. Note that T96S denotes a mutant whose Thr96 is replaced with Ser. It appeared that substitution of Thr96 with Ser and Val retained 90% and 45% of TH activity, respectively, while it becomes inactive when substituted with Ala. For Thr98, Ser substitution had no significant influence on TH, whereas it becomes less than 20% by Val and Ala substitutions. These results suggest that hydroxyl group rather than methyl group of Thrs are essential for TH activity of AFPII, which is quite distinct from the other types of AFP.

• 低温生物工学会の論文
• 2008-08-21

著者

• KONDO Hidemasa

  Functional Protein Research Group, Research Institute of Genome-based Biofactory, National Institute

• Kondo Hidemasa

  Functional Protein Research Group Research Institute Of Genome-based Biofactory National Institute O

• Yasui Masanori

  Functional Protein Research Group Research Institute Of Genome-based Biofactory National Institute O

• TAKAMICHI Manabu

  Functional Protein Research Group, Research Institute of Genome-based Biofactory, National Institute

• MIURA Ai

  Functional Protein Research Group, Research Institute of Genome-based Biofactory, National Institute

• NISHIMIYA Yoshiyuki

  Functional Protein Research Group, Research Institute of Genome-based Biofactory, National Institute

• TSUDA Sakae

  Functional Protein Research Group, Research Institute of Genome-based Biofactory, National Institute

• Miura Ai

  Functional Protein Research Group Research Institute Of Genome-based Biofactory National Institute O

• Tsuda Sakae

  Functional Protein Research Group Research Institute Of Genome-based Biofactory National Institute O

• Takamichi Manabu

  Functional Protein Research Group Research Institute Of Genome-based Biofactory National Institute O

• Nishimiya Yoshiyuki

  Functional Protein Research Group Research Institute Of Genome-based Biofactory National Institute O

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• 1. Hydroxyl groups of threonines contribute to the activity of Ca^-depdendent type II antifreeze protein
• Hydroxyl groups of threonines contribute to the activity of : Ca^-depdendent type II antifreeze protein

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