放射性コバルト(Co^<60>)近接照射の子宮頸部癌に及ぼす組織化学的研究
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概要
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Contact irradiation with Cobalt 60 (one treatment 24 hours, 1,000 mch, altogether 4 treatments on alternative days; total radiation 4,000 mch) was administered to 38 clinical patients with cancer of the uterine cervix. Repeated biopsies were done from the cancerous lesion, before, during and after irradiation for altogether 6 times on each patient. Histochemical evaluation of those specimen was done on the basis of 29 various types of metabolic substances and enzymic activations, for the purpose of observing the changes due to irradion therapy in correlation with their morphological changes. Furthermore, this observation was extended to in order to evaluate the radio-sensitivity of the malignant cancerous tissue. Results from the above study were summerized as follows : 1. A rather marked increase of nucleotid RNA, Glycogen, and fatty substance was noted in the cancer cells following irradiation, while marked decrese or diminishing affect of the enzymic activation was seen in DNA, protein bound SH base, Vitamine B_1, Isocitrate dehydrogenase, Malate dehydrogenase, NAD-(DPN)-Diaphorase and Esterase. 2. Increase of cancer cell nucleotid RNA and appearance of the large granules or their increase in the protoplasma RNA, Glycogen and fatty substance, were closely correlated with degenerative changes in the cancer cells due to irradiation. 3. The connective tissue in the stroma of the cancerous tissue which became loose by irradiation, was found to have an increase in quantity or enzymic activation of α-amino base, fatty substance, ribonucleotidase and phosphatase, while decrease in quantity or weakening in enzymic activity was noted on Vitamine B_1, Isocitrate dehydrogenase, Malate dehydrogenase and NAD (DPN)-diaphorase. Some of the connective tissue which showed morphological evidence of proliferative changes, also displayed an increase of enzymic activity of 5'-nucleotidase, and adenosinetriphsphatase. 4. Changes of metabolic substances and enzymic activity due to irradiation found in the stroma of the cancer tissue, have a very close correlation with the destructive or reconstructive changes in that tissue. Therefore these changes may indicate affect of irradiation therapy to the particular cancerous lesion and can become important in deciding prognosis of the malignancy. 5. Degenerative changes due to irradiation occuring in the cancerous parenchymatous cells, produce an appearance of enzymic active hysteocytic wandering cells. 6. Response of cancer cells to irradiation was found to be most sensitive in Group IV of Cordua's maturity classification of Group IV to VI ; & γ-, β-groups were found to be most sensitive among α-, β-, γ-groups classified by grade of infiltration.
- 千葉大学の論文
- 1965-05-28