組織培養におけるインフルエンザ・ウイルスの増殖について : Roller tubeを使用した基礎的事項の検討

概要

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Influenza A virus (PR-8) was cultivated in roller tube with minced chorioallantoic membrane of embryonated egg, and the multiplication of the virus was followed in terms of hemagglutination (HA) titer. Results were as follows ; Each tube showed constantly uniform titers. Long-time HA curves were obtained through relay of several tubes. Multiplication of the virus on roller tubes showed similar sequences to multiplication in chorioallantoic cavities of embryonated eggs, irrespective of inoculum doses. For the cultivation of influenza virus, minced chorioallantoic membrane seemed to give the results as compared to minced bodies or minced brains of chick embryo. HA activity of influenza virus was not very stable. Addition of potassium chloride 0.6 grm. per liter to the Tyrode's solution increased its stability, whereas calcium chloride did not. From result of examination, in Fig. 6. HA curves showed like a step by increased potassium chloride, it seems that unevenness of HA curves except one step growth of virus is caused by lability of HA activity, Tyrode's, Hanks' and Earle's as nutrient solution, were nearly equal for cultivation of influenza virus. Roller tube method gave HA titers approximately four to eight times higher than stationary method.
1962-03-28