無菌飼育海〓の血清殺菌力,白血球食菌能及び溶血性補体に関する研究
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概要
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Experiments were carried out in guinea pigs raised in an germ-free environment, using the apparatus HMC-2 type which was developed in our department, to study bactericidal power of the sera and phagocytic power of the leukocytes, to determine titers of hemolytic complement, and to investigate changes in these values resulting from infection with the organisms of Escherichia coli group and Lactobacillus group, singly or in combination. The following observations were made: 1. The sera of germ-free guinea pigs, when infected with the organisms of Lactobacillus acidophilus, had a lower bactericidal power against strain 0-901 of Salmonella typhi than did those of the germ-free guinea pigs infected with strain F 6 of Esch. coli. The sera of the latter were nearly as powerful against the bacteria as those of conventional guinea pigs. 2. Bactericidal power of the sera of the germ-free guinea pigs infected with strain Ml of Esch. coli and the organisms of Lactobacillus bifidus in combination was lower than that of the sera of the germ-free guinea pigs infected with strain F 6 of Esch. coli singly. Bactericidal power of the former, however, was a little higher than that of conventional guinea pigs. 3. The leukocytes of germ-free guinea pigs had a lower phagocytic power against Terajima strain of Staphylococcus aureus than did those of conventional guinea pigs. Phagocytic power of the leukocytes of the germ-free guinea pigs infected with strain M1 of Esch. coli and the organisms of Lactobacillus bifidus in combination was higher than that of germ-free guinea pigs, but was lower than that of conventional guinea pigs. 4. Opsonic action of the sera of germ-free guinea pigs was less powerful than that of conventional guinea pigs except in one case. 5. The titers of hemolytic complement of the sera of conventional guinea pigs tended to increase with the passage of time during a period of growth. In the case of germ-free guinea pigs, however, the increase was delayed, and the values tended to show irregular fluctuations.6. The effect of initial infection of germ-free guinea pigs with the bacteria on the titers of hemolytic complement was obscure.
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