組織培養法によるリフト・バレー熱ウイルスの増殖と宿主細胞の形態学的変化について

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概要

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• Rift Valley fever has been known as a epizootic viral disease found in East Africa. The agent does cause acute liver necrosis infecting efficiently on such a laboratory animal as mouse. In our laboratory an attempt has been made to employ cultured cells as a susceptible host for the virus infection. HeLa cells were found to sustain the multiplication of pantropic Rift Valley fever virus. Subcultures were performed through more than 22 passages. The pantropisrn of the virus has not been lost even at the end of these passages. By titrating the virus liberated from infected cells, it was determined that the virus had about 6 hours of latent period in these cells. A long time after release of the virus, infected cells showed degeneration, i. e. pycnosis and marked formation of cytoplasmic projections, subsequently detaching from the glass surface. Neither the cytoplasmic nor intranuclear inclusion was found in any phase of virus multiplication In the early phase of infection, however, the number of so called nucleolar bodies was observed to diminish to some extent. Since this change was also found after the inoculation of heat-inactivated virus, it seems to be justified to regard that the change was caused primarily by the virus or its genie potency. Thus the author pointed a probable involvement of the functions of nucleolar bodies which lately have been considered to take an important role in syntheses of proteins and nucleic acids, into the metabolism of infected cells.

• 千葉大学の論文

著者

• 森 亘敬

  千葉大学医学部細菌学

関連論文

• 34. リフト・バレー熱ウイルスの向神経牲変異に関する実験(第36回千葉医学会総会,第5回千葉県医師会学術大会連合大会演説要旨)
• 組織培養法によるリフト・バレー熱ウイルスの増殖と宿主細胞の形態学的変化について

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