屎尿消化に於けるClostridium菌族の研究 : 附 所謂メタン菌に関する研究
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The digestion of night-soil in a digestion tank mainly depends upon the decomposition of solids by the action of anaerobic bacteria. The author carried out some experiments on anaerobic bacteria, especially the organisms of Clostridium and methane-producing bacteria found in samples of night-soil. The studies were performed with a view to contributing to fundamental studies on gas production in the digestion of night soil. 1. Of anaerobic bacteria found in raw night-soil, digested sludge and supernatant in a digestion tank on a pilot plan, the organisms of Clostridium accounted for the majority. They were always detected in larger numbers in digested sludge than in supernatant, and there was little change in their numbers during the whole period of experiments. 2. When 11 strains of Clostridium isolated from night-soil were implanted in Prevot's methane-producing mediums, none of them were shown to be capable of producing methane by itself. There was, however, a production of methane in one medium inoculated with one of the series of sets of 5 strains selected at random from among the 11 strains, the amount produced representing 4.8 per cent of the total amount of gases produced. When each one of these 11 strains was implanted in a Laigret's medium separately, there was a production of methane in two mediums, representing 7.4 and 5.1 per cent, respectively, of the total amounts of gases produced. All this happened when inoculation of the medium with the strains under study was repeated over and over again. 3. Barker's so-called methane-producing bacteria were identified in night-soil, but it was impossible to obtain their pure cultures. Heuckelekian's method for enumeration of methane-producing bacteria is of value for estimation of the activity of methaneproducing bacteria in methane fermentation of night-soil.
- 千葉大学の論文
- 1959-01-28
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