新生児に於ける大腸菌の由来に関する研究
スポンサーリンク
概要
- 論文の詳細を見る
In an effort to throw light on the process by which the flora of the coli group organisms is formed in human intestines, the author carried out experiments on the culture of the feces of 31 babies every day since immediately after their birth. The author also performed investigations on the relation between the coli group organisms found in the feces of babies and those found in the feces and vagina of their mothers. Part 1. The Relation between the Coli Group Organisms found in Mothers and those found in Newly-born Babies. The feces of 21 newly-born babies collected every day for one week since immediately after birth and those of 21 mothers collected on the day, or the day after, delivery of their babies were cultured in E.M.B. and Endo's mediums. The coli group organisms thus detected were not only studied from the standpoint of morphology and biology but also tested serologically by Kauffman's method, especially by agglutination tests of 0 agglutinin. 1) Detection of Coli Group Organisms in Newly-born Babies. Of the 21 cases under studies, 17 cases were proved to be positive for coli group organisms on the first day following their birth. Coli group organisms, were also detected in the feces of the 4 cases on the second and the third days. Investigations have disclosed that 65 % of organisms detected were identified as Eschelichia coli, while the balance belonged to irregular type, intermediate:, type. and Klebsiella. 2) Coli Group Organisms found in Newly-born Babies a. How they lodged in the Intestines. The same species of coli group organisms as were initially found have been detected consecutively throughout the entire period of investigations in 11 cases out of the total of 17 cases. E. coli showed a persistent tendency to lodge in the intestines. b. Their Species and Numbers. Of all the coli group organisms detected (224 strains), E. coli (164 strains) formed 73.6 %, Klebsiella 19.2 % and intermediate type 3.1 %. The percentages were more or less similar to those of the coli group organisms found in mothers. 3) Agglutination of the coli group organisms found in Newly-born Babies caused by serum immunized against the coli group organisms found in their Mothers. Of the several species of the coli group organisms found in the feces, one strain assumed to be the most prominent had been singled out for each of the 18 mothers. The serum of the rabbit immunized against each of the 18 strains was prepared Then, O agglutination tests were carried out between the serum thus prepared and strains of coli group organisms detected in the respective baby consecutively for one week. The serum agglutinated the coli group organism found in the baby in 4 combinations of mother and baby out of a total of 18 combinations tested. The observations that the presence of coli group organisms in the newly-born babies was proved at the early stages and that the same species of organisms as were found in their mothers were detected in 4 cases out of 18 cases under investigations would indicate that babies are constantly exposed to contamination with coli oroup organisms after their birth and mothers are possible sources of the infection.姫art 2. The Relation between the Coli Group Organisms found in Mothers's Vagina and those found in the Newly-born Baby The secretions were collected by swab from the vagina of 10 hospitalized pregnant women and were cultured in the same manner as was described in Part 1. When the presence of coli group organisms was proved in the medium, the correlation between the coli group organisms found in mother's vagina and her baby was investigated from the standpoint of serology and biology. 1) Four strains of coli group organisms were isolated from the vagina in 3 cases out of the 10 cases under investigations. They consisted of 3 strains of E. cold and I strain of Klebsiella. The presence of coli group Organisms in the feces of the babies born of those 3 mothers was . proved on different days after birth: One baby on the first day, another baby on the second day and still another baby on the third day. In the days elapsed before coli group organisms were found in the feces, there was no difference between those 3 babies and other newly-born babies in whose mothers the presence of the organism in _the vagina had not been proved. 2) O aggulutination tests performed between the serum of the rabbit immunized against the coli group organisms found in mother's vagina and the coli group organism found in her Newly-born baby have revealed that the former agglutinated the latter to the terminal value in 1 combination of mother and baby out of the 3 combinations tested, proving that the coli oroup grganisms used for the tests were of the same species. 3) The observations that the same species of coli group organisms were found in the feces of mother and baby in 4 combinations out of 18 combinations investigated, and that the same species were found in the vagina of mother and the feces of baby in 1 combination out of 3 combinations would indicate the possibility that mother might be the leading source of the infection.
- 千葉大学の論文
著者
関連論文
- 16)尿中排泄性腺刺激ホルモン量をめぐつての排卵機序の考察(第375回千葉医学会例会産婦人科分科会)
- 15. コルセツトの人体に対する影響(第422回千葉医学会例会 衛生学公衆衛生学教室連合会)
- 新生児に於ける大腸菌の由来に関する研究
- 13. 新産児大腸菌の由来に関する研究(I.一般演説,谷川教授開講十周年記念,第329回千葉医学例会,衛生学教室例会連合会演説要旨)