Nocardiaの産生する抗生物質Nocardorubinの研究
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概要
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1. Morphological and taxonomical studies on the microorganism which produce nocardorubin was investigated, and the strain was confirmed to be included in genus nocardia. As the strain was found unlike any other species of that genus, it was considered to be a new species and named Nocardia narashinoensis Nov. sp. 2. Nocardorubin is obtained by extracting with butanol from the acidified culture broth and by extracting with acetone from the mycelia. Nocardorubin is strongly bacteriostatic against a wide variety of gram-positive bacteria including mycobacterium tuberculosis. Microorganisms which are resistant to penicillin or streptomycin retained same sensitivity to nocardorubin as before. 3. Nocardorubin is a scarlet powder that changes its color, red in alkali and yellow in acid. It fails to react in tests for carbohydrate or protein. Nocardorubin turns black at 180℃. but does not melt at 250℃. The alkaline form of nocardorubin is stable in water and other organic solvents and is not affected by heat in its activity. The acid form, however, is unstable. Nocardorubin is not affected by plasma or other various substances under the examination of the antibiotic activity. Development of resistance of microorganisms against nocardorubin is only slight or almost non-existent in comparison with rapid increase against penicillin and streptomycin. 4. Nocardorubin was compared with six antibiotics obtained from nocardia and other antibiotic pigments which was reported in the literature and was decided to be a new antibiotic pigment. 5. ・In vivo activity of nocardorubin was investigated. The maximum dosis showing no toxic reaction against 12-15g. weight mice was 5 mg. when administered subcutaneouslly and 2 mg. intraperitoneally. The blood concentration of nocardorubin injected intramuscularly against rat reached maximum in 1 hour and the effective concentration was retained more than 4 hours. Nocardorubin was proved to be effective in protecting mice against subcutaneous infection of Diplococcus pneumoniae Type 1.
- 千葉大学の論文
- 1955-01-28