嫌気性線維素分解菌に関する研究 : 第1編 嫌気性線維素分解菌に就て
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In reference to the studies of the procedure of human waste in our laboratory, I have investigated the anaerobic cellulolytic bacteria, on supposition that these bacteria liquefy paper and cellulose substances in human waste. We have been much interested on the problem as to reducing of scum and sediment in accumulated excretions. The author has isolated 51 strains of anaerobic cellulolytic bacteria from sludge of pons and feces of horses etc., and divided them into 3 varieties. ( group K_1, K_2, K_3) Main physiological characters of the bacteria are as follows. 1) Bacteria of group Kt are rods, 2.4 to 6.0 by 0.4 microns, longitudinal curving, occurring singly, no chains. Spores ovale (1.4 by 1.0), terminal, swelling rods. Gram negative, nonmotile. K_2 are rods, 2.0 to 7.0 by 0.4 to 0.7 microns, longitudinale curving, occurring singly, no chains. Spores ovale (2.0 by 1.4), terminal, swelling rods. Gram negative, non-motile. K_3 are rods, 0.7 to 1.0 by 0.4 microns, occurring singly, no chains. Spores ovale (0.6 by 0.4), terminal. Gram negative, non-motile. 2) Cellulose agar deep colonies. (1.2 -0x1.f6620bfff517p+0gar) grayish white, delicate, cottony, filmy, scarcely discernible, the transparent ring for the cellulolyse around the colonies. 3) Cellulose agar surface colonies. (1.2 0x1.5666572206e49p+807gar) grayish white, delicate, filmy, rand irregular, the transparent ring for the cellulolyse around the colonies, smell butyricacid. Cellulose agar surface colonies. (2 0x1.f742065636e65p-505gar) grayish white, little, hemispheric, glossily, the transparent ring for the cellulolyse around the colonies, smell butyricacid. 4) Cellulose nutrient solution. Cellulose is destroyed in 7-14 days and gas bubbles arise. pH of the solution varies from 7.5 to 6.0. 5) Broth. K_1, K_2 : no growth. K_3 : growth and slight sediment, but the solution remains visibly clear. 6) Glucose broth. K_1 : no growth. K_2, K_3 : growth and slight sediment, but the solution remains visibly clear.7) Agar surface colonies. K_1, K_2 : no growth. K_3 : grayish white, hemispheric. 8) Glucose agar surface colonies. K_1 : no growth. K_2 : yellowgrayish white, Plain. K_3 : grayish white, hemispheric. 9) Pepton water. K_1, K_2: no growth. K_3: growth and slight sediment, but the solution remains visibly clear. And indol is produced. 10) Gelatin : not liquefied. 11) Potato agar surface colonies. K_1,K_2 : yellow-grayish white, plain. K_3: white hemispheric. 12) Liver broth. The liver does darken, but the solution remains visibly clear. Occasionally gas bubbles arise. 13) Blood agar surface colonies. K_1, K_2 : brown-grayish white, plain. K_3 : grayish white, hemispheric. 14) Coagulated albumin surface colonies. K_1 : no growth. K_2 : light, yellowish, hemispheric, little. K_3 : transparent, hemispheric, little. 15) Milk. Coagulate and produce acid. 16) Fermenting process of carbohydrates. K_1 : don't ferment except cellulose. K_2 : ferment xylose, arabinose, glucose, fructose, sucrose, maltose, lactose, dextrin, solbule starch, inulin, glycogen and mannitol, and produce acid and :gas. gas. But don't ferment dulcitol. K_3: ferment xylose, arabinose, glucose, fructose, sucrose, maltose, lactose, dextrin, soluble starch, glycogen and mannitol, and produce acid and gas. But don't ferment inulin and dulcitol. 17) K_1, K_2 are obligate anaerobe. K_3 are facultative anaerobe. 18) All grow between pH 6.0 and 8.5. 19) The spores can resist heat at 90℃ for 20 minutes and at 100℃ for 3 minutes. 20) The spores die out at 1 % Liquor cresolis saponatus after 3 minutes and at 2 after 2 minutes.
- 千葉大学の論文
- 1953-11-28