無菌海〓の腸内細菌感染とリンパ節の形態的変化について
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In our department, experiments have heen carried out in animals born and raised in a germ-free environment to study the action of intestinal bacteria on their hosts. As part of this study, the author conducted experiments in germless guinea pigs to study possible influence that intestinal bacteria might exert on development of the lymph system which has been shown to play an important part in protecting the body against infections. Germless guinea pigs born and raised in an equipment, H. M. C.-2 type, developed in our department for germless raising of animals, were infected with Escherichia coli, Lactobacillus acidophilus and enterococcus, either singly or together. Then, their mesenteric and inguinal lymph nodes were studied morphologically, and the results were compared wtih the observations made in guinea pigs born and raised in a normal environment, guinea pigs fed with sterile food, and germless guinea pigs, which served as controls. The study was concentrated on the detection of the secondary nodules, particularly the germinal center. The results, which are summarized as follows, show that intestinal bacteria may be concerned in the development of the lymph nodes. They threw light to some extent on the influence of non-pathogenic intestinal bacteria on the body. 1. In guinea pigs born and raised in a normal environment, the secondary nodules made their appearances 'markedly, and the germinal centers were clearly detected. 2. In guinea pigs fed with sterile food, the appearance of the secondary nodules was marked, as in guinea pigs born and raised in a normal environment. 3. In germless guinea pigs, the lymph nodes were generally rough in appearance, and no secondary nodule was detected. 4. Guinea pigs infected with a strain spore forming bacteria showed good development of the lymph nodes, with marked appearance of both the solid nodules and germinal centers. 5. Germless guinea pigs infected with a small number of a strain of Esch. coli (0-101) and a Iarge number of another strain of E. coli (0-104) long survived. There were only rough solid nodules and no germinal center in their lymph nodes. 6. Germless guinea pigs infected with a large number of the bacteria (0-101) and a small number of the bacteria (0-104) died in a few days, probably because toxins of the former were fatal. No secondary nodule was detected, and the pathologic picture rather resembled the picture of the cells undergoing degeneration. 7. Guinea pigs infected with a mixture of the bacteria (1-101) and L. acidophilus presented two different pictures: in one of them there was no germinal center, but a small number of the solid nodules and the nodules of a transitory type were detected, while in the other the observation made was similar to that made in guinea pigs infected with a large number of the bacteria (0-101) and a small number of the bacteria (0-104). 8. In guinea pigs infected with L. acidophilus, neither the germinal center nor the solid nodule was detected. In over-all development of the lymph node, they were far superior to germless guinea pigs, though inferior to guinea pigs born and raised in a normal environment. 9. In guinea pigs infected with a large number of enterococcus and a small number of the bacteria (0-104), no germinal center but a small number of very rough nodules were detected. Over-all development of the lymph node was good. 10. Guinea pigs infected with a large number of the bacteria (0-101) and a small number of enterococcus died in no time, probably because toxins of the former were fatal. Observation made in them was similar to that made in guinea pigs infected with a large number of the bacteria (0-101) and a small number of the bacteria (0-104).
- 千葉大学の論文
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