Production of β-xylanase by a Thermomyces lanuginosus MC 134 mutant on corn cobs and its application in biobleaching of bagasse pulp(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
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The production of hemicellulases by Thermomyces lanuginosus SK using oatspelts xylan was examined during submerged cultivation. A high level of extracellular xylanase (346±10Uml^<-1>) production was observed on the fifth day; however, accessory enzyme levels were low. T. lanuginosus SK was further subjected to UV and N-methyl-N-nitro-N-nitrosoguanidine mutagenesis. The T. lanuginosus MC 134 mutant showed a 1.5 fold increase in xylanase production on oatspelts xylan, compared to the wild type strain. Xylanase production was further enhanced to 3299±95Uml^<-1> by using corn cobs under optimized growth conditions. A reduction in xylanase production was observed in a 5L fermenter. Also, the biobleaching efficiency of crude xylanase was evaluated on bagasse pulp, and a brightness of 46.07±0.05% was observed with the use of 50U of crude xylanase per gram of pulp. This brightness was 3.6 points higher than that of the untreated samples. Reducing sugars (25.78±0.14mg g^<-1>) and UV-absorbing lignin-derived compound values were considerably higher in xylanase-treated samples. T. lanuginosus MC 134 has a potential application in the pulp and paper industries.
著者
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Kumar Kuttanpillai
Department of Biotechnology and Food Technology, Faculty of Applied Sciences, Durban University of T
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Manimaran Ayyachamy
Department of Biotechnology and Food Technology, Faculty of Applied Sciences, Durban University of T
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Permaul Kugen
Department of Biotechnology and Food Technology, Faculty of Applied Sciences, Durban University of T
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Singh Suren
Department of Biotechnology and Food Technology, Faculty of Applied Sciences, Durban University of T
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Singh Suren
Department Of Biotechnology And Food Technology Faculty Of Applied Sciences Durban University Of Tec
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Permaul Kugen
Department Of Biotechnology And Food Technology Faculty Of Applied Sciences Durban University Of Tec
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Kumar Kuttanpillai
Department Of Biotechnology And Food Technology Faculty Of Applied Sciences Durban University Of Tec
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Manimaran Ayyachamy
Department Of Biotechnology And Food Technology Faculty Of Applied Sciences Durban University Of Tec
関連論文
- Production of β-xylanase by a Thermomyces lanuginosus MC 134 mutant on corn cobs and its application in biobleaching of bagasse pulp(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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