In vitro, in vivoにおけるB型肝炎ウイルス遺伝子型間の違い(受賞論文,平成19年度名古屋市立大学医学会賞)

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概要

• 論文の詳細を見る

• Hepatitis B virus (HBV) genotypes induce liver disease of distinct severity, but the fundamental virological differences are not well defined. In our recent studies using Huh7 cells and chimeric mouse harboring human hepatocytes, we demonstrated the particular characteristics of each genotype although it was general that virological properties of HBV genotypes were almost similar to each other. We have cloned 1.2-fold the HBV genome of different genotypes/subgenotypes from serum samples. HBV DNA levels in cell lysates were the highest for HBV/C and B and the lowest for HBV/A and D. HBe antigen and HB core protein were excreted in a trend similar to that of HBV DNA with similar differences. Secretion of HB surface antigen, however, was the most abundant HBV/A followed by B, C and remotely by D, which was consistent with mRNA levels. Chimeric mouse with the liver replaced for human hepatocytes were inoculated with virion passed in mouse. HBV DNA levels in their sera were higher for HBV/C than A by 2 logs during 4-7 weeks after inoculation. Virological differences among HBV genotypes were demonstrated both in vitro and in vivo. These characteristics might influence HBV infection in clinical and epidemiological setting.

• 名古屋市立大学の論文

著者

• 杉山 真也

  名古屋市立大学大学院医学研究科ウイルス学 肝疾患センター

関連論文

• ウイルス感染と肝発癌 HBV感染による肝発癌 HBVの分子生物学 (肝癌--基礎・臨床研究のアップデート) -- (肝癌の発生・進展機序)
• B型肝炎ウイルス感染による肝細胞傷害の機序 : HBV遺伝子型と病原性
• In vitro, in vivoにおけるB型肝炎ウイルス遺伝子型間の違い(受賞論文,平成19年度名古屋市立大学医学会賞)

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