キャピラリー液体クロマトグラフィーのためのマイクロフロー制御技術の開発
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概要
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Capillary liquid chromatography (LC) saves energy and waste, and possesses several advantages over conventional LC ; nevertheless it has not been very much popularized since its appearance due to the fact that the column efficiency of the capillary at this moment is not dramatically higher than that of conventional LC. The present research focused on developing versatile capillary LC systems using commercially available low dead-volume micro unions and valves, allowing simple postcolumn-controlled stepwise-gradient elution, on-line sample enrichment (on- and precolumn enrichments) and recycle separation with relatively superior efficiency and tolerable band broadening. The on-column enrichment gave a 50-fold improvement in sample injection, while the precolumn allowed injection of up to 5000-fold, which is so far the highest enrichment factor that has been reported. These systems were successfully applied to the analysis of environmental as well as drinking water samples. Alternatively, an alternate-pumping recycle chromatography system was newly-designed using two monolithic silica capillary columns, and baseline separation of benzene and deuterated benzene was achieved after 5 cycles when the system attained 160000 theoretical plates. Additionally, the development of a novel glass-bead-based immobilized-enzyme micro column for simple and swift on-line protein digestion and its peptide separation by reversed-phase HPLC was also described.
- 社団法人日本分析化学会の論文
- 2008-10-05