83(PA3-3) アブラナ連植物の産生するファイトアレキシン(ポスター発表の部)

概要

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The tribe Brassiceae (Cruciferae) includes many vegetables of economic importance. However, no phytoalexins, antimicrobial compounds synthesized by plants on their exposure to microorganisms, have been isolated. We carefully investigated the conditions for phytoalexin formation from two genera of the tribe, Chinese cabbage (Brassica campestris L. ssp. pekinensis) and daikon (Raphanus sativus L. var. hortensis), and found that the bacterium Pseudomonas cichorii induced the formation of several antifungal compounds not detected in control tissues. Sliced heads of Chinese cabbage were inoculated with P. cichorii and incubated for 3 days. Extraction of the tissue with acetone and separation by repeated chromatography over silica gel and Sephadex LH-20 gave five antifungal compounds (1-5). Analogous treatment of the tissue of daikon resulted in the isolation of eight antifungal compounds (6-13), together with the above compounds 1, 2, and 4. These compounds were shown to be indole derivatives on the basis of spectroscopic studies and synthesis. These are the first phytoalexins from the family Cruciferae and also the first ones containing sulfur atoms.
1987-07-25