Preparation of bone-like apatite coating on mullite ceramics with silicon-ion releasability(<Feature> Ceramics for Biomedical Applications in Asian Countries)
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概要
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A preparation method of bone-like hydroxyapatite (b-HA) coating with silicon-ion releasability on ceramic substrates was investigated for developing scaffolds with high mechanical strength and cellular compatibility for bioreactors. A trace amount of silicon ion has been reported to stimulate the activities of osteoblastic cells. A mullite ceramic substrate was covered with a silica layer derived from water glass through two steps, i.e., heat-treatment at 500℃ for 2h and subsequent acid-treatment using 0.1mol・dm^<-3> HCl solution at 50℃ for 2h, and then coated with nano-sized vaterite particles in monolayer. The monolayer was produced by the balance of the surface potentials between the negatively charged silica layer and the positively charged vaterite. The resulting substrate was homogeneously coated with b-HA layer of about 2μm in thickness after 3d of immersing in an aqueous solution with inorganic ion concentrations of 1.5 times those of simulated body fluid. The b-HA-coated sample was immersed for 7d in culture medium and 260μmol・dm^<-3> of silicon ion was released simultaneously. On the other hand, 130μmol・dm^<-3> of the ion was released from the b-HA-coated sample after heating at 800℃ for 3h. The silicon-ion releasability of the b-HA-coated sample was controllable by the heat-treatment, which was expected to be ascribed to increase in the crystallinity of the b-HA coating and in the durability of the silica layer in culture medium by the heat-treatment. The b-HA-coated samples showed good compatibility with mesenchymal stem cells. The proliferation and osteogenic differentiation of the cells changed on the b-HA-coated samples between before and after the heat-treatment. The b-HA coating with silicon-ion releasability on ceramic substrates is expected as bioreactor scaffolds with the high activity of the cellular proliferation.
- 社団法人日本セラミックス協会の論文
- 2008-01-01
著者
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KASUGA Toshihiro
Graduate School of Engineering, Nagoya Institute of Technology
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Obata Akiko
Dep. Of Frontier Materials Graduate School Of Engineering Nagoya Inst. Of Technol.
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Obata Akiko
Department Of Frontier Materials Graduate School Of Engineering Nagoya Institute Of Technology
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Kasuga Toshihiro
Nagoya Inst. Of Technol. Dep. Of Frontier Materials Gokiso
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Kasuga Toshihiro
Department Of Frontier Materials Graduate School Of Engineering Nagoya Institute Of Technology
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OBATA Akiko
Graduate School of Engineering, Nagoya Institute of Technology
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OGURA Nobuo
Graduate School of Engineering, Nagoya Institute of Technology
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Ogura Nobuo
Graduate School Of Engineering Nagoya Institute Of Technology
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