The infl uence of serial passage on cyclic tensile strain-induced osteoprotegerin synthesis from normal human osteoblasts in vitro
スポンサーリンク
概要
- 論文の詳細を見る
The application of mechanical stress is known to be the unique method of analyses on bone metabolismand remodeling in vitro and in vivo. We have investigated on the effects of the application of cyclic tensile strain（CTS） on the syntheses of bone metabolic factors from the normal human osteoblasts in vitro. In this study, we foundthat diff erent passage numbers aff ected synthesis of CTS-induced factors from osteoblasts in vitro. Osteoblasts werestimulated with the application of CTS for 3 days, 4 hrs a day. Then, it was found that the application of CTS increasednitric oxide（ NO） production, the expression of cyclooxygenase 2（ Cox-2） mRNA, and synthesis of osteopontin（ OPN）from osteoblasts during serial passage. However, CTS-induced osteoprotegerin（ OPG） synthesis from osteoblasts wasshown to change at diff erent passage numbers. In short, increasing at the 3rd passage, CTS-induced OPG synthesis fromosteoblasts decreased at the 5th passage. While, though soluble RANKL（ sRANKL） release by the application of CTSdecreased at 1st passage and did not change at the 5th passage, the expression of receptor activator of nuclear factor-κBligand（ RANKL） mRNA increased in osteoblasts at the 5th passage, decreasing in those at the 3rd passage. The increaseon OPN synthesis the expression of Cox-2 mRNA, and NO production from osteoblasts by the application of CTS wasnot aff ected during serial passage. Analyzing on the activity of the p38 mitogen-activated protein kinase（ p38 MAPK）in osteoblasts with or without the application of CTS, the p38 MAPK activity in osteoblasts at the 3rd passage inhibitedin compared with that at the 3rd passage. From these results, we should use osteoblasts at the 1st passage whenanalyzing on CTS-induced OPG synthesis with normal human osteoblasts. It is suggested that the regulation of CTSinducedOPG synthesis might be aff ected by aging of osteoblasts.
- 弘前大学の論文
著者
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Kubota Kohsei
Department Of Dentistry And Oral Surgery Hirosaki University School Of Medicine
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Kubota Kosei
弘前大学 医学部脳血管病態学
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Kubota Kosei
弘前大学医学部附属脳神経血管病態研究施設 脳血管病態学
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Sashinami Hiroshi
Department Of Microbiology And Immunology Hirosaki University Graduate School Of Medicine
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Sashinami Hiroshi
弘前大学医学部附属脳神経血管病態研究施設 脳血管病態学
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Kimura Hiroto
Department Of Dermatology Hirosaki University School Of Medicine
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Kusumi Akinori
The Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine
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Sakaki Hirotaka
The Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine
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Kusumi Tomomi
Pathology and Bioscience, National University Corporation, Hirosaki University Graduate School of Me
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Nakagawa Hiroshi
The Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine
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Kubota Kosei
The Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine
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Oda Mitsuo
The Department of Oral Anatomy, Showa University School of Dentistry
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Satoh Hisashi
The Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine
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Kimura Hiroto
The Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine
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Oda Mitsuo
The Department Of Oral Anatomy Showa University School Of Dentistry
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Kusumi Tomomi
Pathology And Bioscience National University Corporation Hirosaki University Graduate School Of Medi
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Satoh Hisashi
Hirosaki Univ. Graduate School Of Medicine Hirosaki Jpn
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Kusumi Akinori
The Department Of Dentistry And Oral Surgery Hirosaki University Graduate School Of Medicine
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Nakagawa Hiroshi
The Department Of Dentistry And Oral Surgery Hirosaki University Graduate School Of Medicine
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Sakaki Hirotaka
Department Of Dentistry And Oral Surgery Hirosaki University School Of Medicine
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