モルモット皮膚を用いた炎症評価法 : アスコルビン酸リン酸エステルによるクロトン油誘起性炎症の抑制効果

概要

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In vivo evaluating model is accepted as a powerful tool for determining pharmaceutical effects and safe dosage for clinical use. We established the animal model for evaluating the inflammation caused by reactive oxygen species (ROS). Croton oil (CRO) was topically applied to induce ROS-derived inflammation on the guinea pig skin. Visual inspection of the erythema was performed on 24h after application to evaluate the CRO-induced skin response on a score of one to six. Ascorbate-2-phosphate (AP), ascorbic acid derivative, has been reported to be a potent antioxidant action. To examine its effect on this model, AP was applied topically after CRO application. The inflammation score was significantly reduced by 32-52% of that in the control group. Histological evaluation revealed that AP also decreased the inflammatory thickening and the expression of the oxidative stress marker, 8-hydroxy-2'-deoxyguanosine (8-OHdG) in the skin. These results show the CRO-induced inflammation model developed here is useful tool for evaluating anti-inflammatory and antioxidant effects.
2008-02-25