Soluble Liver Antigen/Liver and Pancreas, an Antigen in Autoimmune Hepatitis Patients : Influence on Selenocysteine Synthesis and its Complex with HSP70
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概要
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Autoimmune hepatitis (AIH) serum contains autoantibodies against soluble liver antigen/liver and pancreas (SLA/LP), a protein that interacts with the selenocysteine (Sec)-tRNA^<Sec>. We investigated the influence of AIH serum on Sec production. Two (#3 and #4) of four AIH sera inhibited its synthesis, with serum #4 showing the strongest inhibition. This did not parallel the level of antibodies to SLA/LP (anti-SLA/LP) in sera, because the titer in serum #3 was higher than that in serum #4. Thus, we found that AIH inhibited Sec synthesis but we could not conclude that this inhibition depended on an autoantibody against SLA/LP. To clarify the function of SLA/LP, we prepared an antibody against an SLA/LP peptide. Inhibition of Sec synthesis by this anti-peptide antibody was incomplete and sometimes did not occur. Absorption of autoantibodies in the AIH serum with the SLA/LP protein did not have any effects on Sec synthesis by Sec synthase (SecS) which converts Seryl (Ser)-tRNA^<Sec> to Sec-tRNA^<Sec>. We then used SLA/LP instead of active bovine SecS but we could not detect SecS activity in SLA/LP itself. Next, we studied the distribution of SLA/LP in HEK293 cells transfected with an SLA/LP expression vector. Immunofluorescence microscopy detected SLA/LP expression in the cytoplasm, but not the nucleus of HEK293 cells. Higher levels of SLA/LP in the cytosol did not correlate with higher Sec production, which remained identical to mock-transfected cells. Interestingly, we found that SLA/LP formed a complex in the cytosol of HEK293 cells. This complex was purified and the components analyzed using Nano-LC and MS/MS, which we identified as HSP70 and tubulin. Altogether, our data suggest that SLA/LP may play an indirect role to produce Sec in the selenoprotein synthesis machinery.
- 公益社団法人日本薬学会の論文
- 2007-12-01
著者
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Isobe Toshiaki
Faculty Of Sciences Tokyo Metropolitan University
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Tanaka Yuta
Graduate School Of Pharmaceutical Sciences Nagoya City University
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Osaka Takashi
Graduate School of Pharmaceutical Sciences, Nagoya City University
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Hattori Asuka
Graduate School of Pharmaceutical Sciences, Nagoya City University
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Murakami Yasuko
Jikei University School of Medicine
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Matsufuji Tamiko
Jikei University School of Medicine
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Matsufuji Senya
Jikei University School of Medicine
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Shinkawa Takashi
Faculty of Sciences, Tokyo Metropolitan University
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Mizutani Takaharu
Graduate School of Pharmaceutical Sciences, Nagoya City University
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Shinkawa Takashi
Faculty Of Sciences Tokyo Metropolitan University
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Osaka Takashi
Graduate School Of Pharmaceutical Sciences Nagoya City University
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Hattori Asuka
Graduate School Of Pharmaceutical Sciences Nagoya City University
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Hattori Asuka
Graduate School Of Engineering Nagoya University
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Mizutani Takaharu
Graduate School Of Pharmaceutical Sciences Nagoya City Univ.
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- Soluble Liver Antigen/Liver and Pancreas, an Antigen in Autoimmune Hepatitis Patients : Influence on Selenocysteine Synthesis and its Complex with HSP70
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