Optimization of an HPLC Method for Determination of Gabapentin in Dosage Forms through Derivatization with 1-Fluoro-2, 4-dinitrobenzene

概要

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A rapid, sensitive and accurate high performance liquid chromatography with UV detection method was developed and validated for the quantification of gabapentin in dosage forms. Gabapentin was quantified after precolumn derivatization with 1-fluoro-2, 4-dinitrobenzene. Amlodipine was used as an internal standard. The chromatographic separation was carried out on a Nova-Pak C_<18> column using a mixture of acetonitrile-sodium dihy-drogenphosphate (pH 2.5 ; 0.05M) (70 : 30, v/v) as mobile phase with UV detection at 360nm. The method was linear over the range of 10-500μg/ml of gabapentin (r^2>0.999). The within-day and between-day precision values were in the range of 0.86-1.11%. The method was successfully used for quantitative determination and dissolution rate study of Neurontin capsules.
公益社団法人日本薬学会の論文
2007-10-01