生物試料の多元素分析と化学形態別分析に関するメタロミクス研究(博士論文要録)

概要

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In relation to metallomics research, the multielement determination and chemical speciation of major-to-ultratrace elements in biological samples were investigated by ICP-AES and ICP-MS with the aid of HPLC. First, the determination of multielements in human blood serum donated from healthy volunteers and disease patients was carried out. As a result, it has been suggested that a multielement analysis of serum may be available for the medical diagnosis of specific diseases. Next, the chemical speciation of mercury and the assay of ALP in salmon egg cell cytoplasm were performed using CHAPS-coated ODS and the SEC columns. The existence of some protein-binding mercury and cysteine-binding mercury in salmon egg cell cytoplasm was confirmed. The analytical results indicate that Hg in salmon egg cell cytoplasm binds with proteins containing selenocysteine and/or cysteine residues. In an ALP assay study, the existence of hydrophilic ALP and hydrophobic conjugated proteins of ALP in salmon egg cell cytoplasm was found. Finally, a phosphatidylcholine-coated ODS column was newly developed and applied to a speciation analysis of arsenic compounds in human blood serum. As a result, it was found that arsenite dosed in the vein of a leukemia patient for therapeutic treatment was converted mainly to monomethylarsonic acid and dimethylarsinic acid.
2007-10-05