低分子有機酸のイオン排除液体クロマトグラフイー/質量分析法
スポンサーリンク
概要
- 論文の詳細を見る
Ion exclusion liquid chromatography/mass spectrometric (LC/MS) analytical method of low molecular organic acids has been developed. Successful separation and detection of a mixture of acetic, propionic, butyric, glycolic, lactic, 2-hydroxybutyric, malonic, succinic, glutaric, tartaric and citric acids have been performed by on an improved ion exclusion column, which is semi-rigid styrene-divinylbenzene copolymer-based H-type cation exchange resin (ULTRON PS-80H), and electrospray ionization mass spectrometry (ESI-MS). Formic acid or acetic acid was used as a mobile phase to separate the above carboxylic acids within 15 min. The ESI interface was used in both positive and negative ionization mode for LC/MS. ESI produced reasonable signals from positive ions ([M+NH_4]^+) of acetic, propionic and butyric acids and from negative ions ([M-H]-) of glycolic, lactic, 2-hydroxybutyric, malonic, succinic, glutaric, tartaric and citric acids. The effects of ionization parameters, source temperature, capillary voltage and cone voltage, on sensitivity and linearity were examined. Linear plots of peak area versus concentration were obtained over the ranges 2.0-40 mM for acetic, propionic and butyric acids, and 0.5-40 μM for the others for MS detection. The detection limits of the target carboxylic acids calculated at S/N=3 ranged from 0.02-1.6 mM for acetic, propionic and butyric acids, and from 0.09-0.44 μM for the others. The reproducibility of retention times and peak areas were 0.55-1.25% and 0.85-2.45%, respectively. The effect of a polar organic solvent, methanol or acetonitrile, which was used as an additive to mobile phase, was also examined. Inscrease of the detection sensitivities of both succinic and citric acids was observed when methanol was used as the additive at the concentration of 5-10% (v/v).
- 高知大学の論文
- 2007-03-30
著者
関連論文
- 低分子有機酸のイオン排除液体クロマトグラフイー/質量分析法
- 低分子有機酸の2-ニトロフェニルヒドラジド誘導体の液体クロマトグラフィー/質量分析法
- ジオール化合物の液体クロマトグラフィー/質量分析法におけるm-アミノフェニルボロネート誘導体の役割