Characterization of Efficient Aerobic Denitrifiers Isolated from Two Different Sequencing Batch Reactors by 16S-rRNA Analysis(ENVIRONMENTAL BIOTECHOLOGY)
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概要
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By adopting two sequencing batch reactors (SBRs) A and B, nitrate as the substrate, and the intermittent aeration mode, activated sludge was domesticated to enrich aerobic denitrifiers. The pHs of reactor A were approximately 6.3 at DOs 2.2-6.1 mg/l for a carbon source of 720 mg/l COD; the pHs of reactor B were 6.8-7.8 at DOs 2.2-3.0mg/l for a carbon source of 1500mg/l COD. Both reactors maintained an in fluent nitrate concentration of 80 mg/l NO_3^--N. When the total inorganic nitrogen (TIN) removal efficiency of both reactors reached 60%, aerobic denitrifier accumulation was regarded completed. By bromthymol blue (BTB) medium, 20 bacteria were isolated from the two SBRs and DNA samples of 8 of these 20 strains were amplified by PCR and processed for 16SrRNA sequencing. The obtained results were analysed by a Blast similarity search of the GenBank database, and constructing a phylogenetic tree for identification by comparison. The 8 bacteria were found to belong to the genera Pseudomonas, Delftia, Herbaspirillum and Comamonas. At present, no Delftia has been reported to be an aerobic denitrifier.
- 2007-06-25
著者
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Wang Ping
Department Of Chemical And Environmental Engineering Beijing Technology And Business University
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Xiang Mufei
Department Of Chemical And Environmental Engineering Beijing Technology And Business University
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Li Xiuting
Department of Chemical and Environmental Engineering, Beijing Technology and Business University
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Zhai Qian
Department of Chemical and Environmental Engineering, Beijing Technology and Business University
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Zhai Qian
Department Of Chemical And Environmental Engineering Beijing Technology And Business University
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Li Xiuting
Department Of Chemical And Environmental Engineering Beijing Technology And Business University
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Wang Ping
Department of Applied Physics, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan
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