The Potential of Cell Suspension Cultures of Daucus carota L. as a Source of Isotope Labelled Gibberellins. I. Metabolism of [^3H]GA_5(Biological Chemistry)

概要

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Gibberellin A_5 (GA_5), which is not known to be native to carrot (Daucus carota L.), was fed as either high specific activity [1-^3H]GA_5 (HSA, 3.2 Ci/mmol) or low specific activity [1,2-^3H]GA_5 (LSA, 74 mCi/mmol) to cell suspension cultures of carrot. Gibberellins A_1, A_3, A_6, A_8 and their glucosyl conjugates were found as metabolites of HSA [^3H]GA_5, as were GA_<22>, GA_<29> and GA_5 glucoside and glucosyl ester, with all tentative identifications based on retention times on reversed phase C_<18> HPLC with radiocounting. From LSA [1,2-^3H]GA_5 feeds, GA_1, GA_3, GA_6, GA_8 and GA_<29> were identified by GC/SIM. For cells or medium, only 24 to 27%, respectively, of [^3H]GA_5 was metabolized; major metabolites were [^3H]GA_5 glucosyl conjugates (HSA and LSA feeds) and free [^3H]GA_<1/3> (LSA feeds). The system may thus be useful for production of labelled Gas and GA conjugates, and especially for conjugates of GA_5 and free GA_6 without dilution by endogenous Gas.
社団法人日本農芸化学会の論文
1988-01-23