A rel Mutation Abolishes the Enzyme Induction Needed for Actinomycin Synthesis by Streptomyces antibioticus(Microbiology & Fermentation Industry)

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概要

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• A relaxed (rel) mutant was found among thirty spontaneous thiopeptin-resistant isolates of Streptomyces antibioticus strain 3720, and actinomycin-producing strain, which showed severely reduced ability to accumulate ppGpp during a nutritional shift-down. The pool size of GTP decreased markedly in the parental strain, but to a lesser extent in the rel mutant. The rel mutant did not show the induction of an enzyme, phenoxazinone synthase, which is involved in the biosyntheses of actinomycin. No negative effect of the rel mutation was observed on a constitutive enzyme, kynurenine formamidase, which also plays a role in actinomycin synthesis. The mutant also failed to produce melamin, but still retained the ability to form aerial mycelium and spores, although the onset of the formation of aerial mycelium was markedly delayed. Neither thephenoxazinone synthase activity nor the kynurenine formamidase activity was affected by ppGpp in vitro. It is suggested tha the stringent response (ppGpp) may be generally essential for the induction of enzymes involved in secondary metabolism.

• 社団法人日本農芸化学会の論文
• 1987-03-22

著者

• Ochi Kozo

  Exploratory Research Laboratories Fujisawa Pharmaceutical Co. Ltd.

関連論文

• A rel Mutation Abolishes the Enzyme Induction Needed for Actinomycin Synthesis by Streptomyces antibioticus(Microbiology & Fermentation Industry)
• Sporulation and Antibiotic Production by Bacillus subtilis Mutants Deficient in Intracellular Proteases

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