アビジン修飾ガラス基板の作製と安定性の比較(<特集>機能性界面と分析化学)

概要

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The formation of avidin sites on the surface of glass slips by chemical modification and an avidin-biotin technique was investigated. In a chemical modification approach, a glass surface was treated with 3-mercaptopropyltrimethoxysilane, on which avidin was introduced by an amine coupling method. In the avidin-biotin technique, biotin sites were formed on glass slips by the patterning of biotin-4-fluorescein on bovine serum albumin (BSA)-modified and BSA adsorbed glass slips by UV irradiation. Avidin-modified glass slips were prepared by treating the biotin sites with an avidin solution. The avidin slips prepared by UV patterning were able to be kept in the dark at 4℃ for at least 5 days, while the chemically modified slips were able to be kept for one day. The preparation of avidin arrays by UV patterning was also demonstrated. The avidin slips appeared to be useful for introducing biomolecules to design a variety of sensing systems.
2007-06-05