低温性Pseudomonas属細菌におけるアデノシンリン酸および核酸への{2-3H}アデニンの取込み

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Growth curves of the psychrotrophic Pseudomonas isolates show an increased slope throughout growth Gn Type 1 strains) or a decreased slope after the stationary phase of growth Gn Type 2 strains), and the levels of adenylate concentrations and changes with growth time in the concentrations in the culture arc different betwccn Type 1 and 2 strains. In order to study whether the psychrotrophic isolates showing different growth curves are composed of different ceotype and moreover the psychrotrophs and the mesophilic Escherichia coLi strain show different adenylate metabolisms, the incorporation of [2_3H] adenine into adenosine phosphates (AMP, ADP, and ATP) and nucleic acids (DNA and RNA) was determined. The incorporation of [2-3 HJ adenine into adenosine phosphates and nucleic acids and the gradient of a straight line given by kinetics of [3H] adenosine phosphate or [3HJ nucleic acid productions and incubation time varied with culture ages, and the variations were peculiar in each of Type 1 and 2 strains. These results suggested that Type 1 and 2 strains are composed of different ecotype. The levels of adenylate concentrations and changes with growth time in the concentrations in the culture were different between the psychrotrophic Pseudomonas isolates and the mesophilic E. coLi strain, and the incorporation of [2-3 HJ adenine into adenosine phosphates and nucleic acids and the gradient of a straight line given by kinetics of [3H] adenosine phosphate or [3H] nucleic acid productions and incubation time varied with culture ages, the variations were peculiar in each of the psychrotrophs and the mesophiles. These results suggested that psychrotrophic Pseudomonas and mesophilic E. coLi have different adenylate metabolims.

低温性Pseudomonas属細菌におけるアデノシンリン酸および核酸への{2-3H}アデニンの取込み

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