Biological Effects of Field Emission-Type X-Rays Generated by Nanotechnology
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概要
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Field emission/X-ray/Nanotechnology/Carbon nanotube. Thermionic emission (TE)-type X-ray generators have been exclusively used in medicine, but there are many difficulties in making these X-ray sources compact. A field emission (FE)-type X-ray generator using carbon nanotubes is a newly-developed compact system that can be as small as several cm in length. Considering the compactness of the equipment, the FE-type X-ray generator may become a useful tool for endoscopic, intracavitary or intraoperative radiotherapy in the future. The aim of this study was to investigate the biological effects of X-rays generated by the FE-type X-ray source in comparison with those of conventional TE-type X-rays. Mouse thymic lymphoma 3SB cells were irradiated by an FE-type X-ray generator developed by our group and a conventional TE-type X-ray source under identical conditions. DNA damage after radiation was detected by foci formation of phospho-H2AX (γ-H2AX). Effect on the cell cycle was analyzed by flow cytometry. Activation of the DNA damage checkpoint was analyzed by immunoblotting. Induction of apoptosis was studied using the TUNEL assay. In terms of induction of DNA damage (DNA double-strand breaks), activation of cell cycle checkpoints (p53 stabilization, p21 induction, Chk1 and Chk2 phosphorylations), and induction of apoptotic cell death, FE-type X-rays were as effective as TE-type X-rays, and FE-type X-rays appeared to be applicable to radiation therapy.
- 2007-03-20
著者
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SHIBAMOTO Yuta
Department of Radiology, Nagoya City University Hospital
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OTSUKA TAKANOBU
Department of Musculoskeletal Medicine, Nagoya City University Graduate School of Medical Sciences
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Nakanishi Makoto
Department of Chemistry, Faculty of Science, Kyoto University
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Shibamoto Yuta
Department Of Radiology Nagoya City University Graduate School Of Medical Sciences
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Shibamoto Yuta
Chubu Radiation Oncology Group
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NAKAZATO Tomoharu
Department of Musculoskeletal Medicine, Nagoya City University, Graduate School of Medical Sciences
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KITA Shigetomo
Graduate School of Engineering, Nagoya Institute of Technology
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OKUYAMA Fumio
Graduate School of Engineering, Nagoya Institute of Technology
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Otsuka Takanobu
Department Of Musculoskeletal Medicine Nagoya City University Graduate School Of Medical Sciences
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Nakanishi Makoto
Department Of Applied Chemistry Faculty Of Engineering Okayama University
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Okuyama Fumio
Graduate School Of Engineering Nagoya Institute Of Technology
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Kita Shigetomo
Graduate School Of Engineering Nagoya Institute Of Technology
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Shibamoto Yuta
Department Of Oncology Chest Disease Research Institute Kyoto University
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Nakazato Tomoharu
Department Of Musculoskeletal Medicine Nagoya City University Graduate School Of Medical Sciences
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Nakazato Tomoharu
Department Of Molecular Engineering Graduate School Of Engineering Kyoto University
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OTSUKA Takanobu
Department of Musculoskeletal Medicine, Nagoya City University, Graduate School of Medical Sciences
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