Turmeric and Curcumin Modulate the Conjugation of 1-Naphthol in Caco-2 Cells(Miscellaneous)
スポンサーリンク
概要
- 論文の詳細を見る
Turmeric, the powdered dry rhizome of the Curcuma longa plant, and curcumin, the major anti-oxidant constituent of turmeric, have been shown to possess chemopreventive activity. To elucidate the possible interaction of turmeric and curcumin with conjugation reactions, which in many cases are involved in the activation of procarcinogens, we measured their effects in the conjugation of 1-naphthol in Caco-2 cells, a human colon carcinoma cell line, within a 24h period. Turmeric exhibits inhibitory activity toward both sulfo- and glucuronosyl conjugations of 1-naphthol at approximately the same levels (IC_<50>=0.24 and 0.29mg/ml, respectively). Curcumin inhibits sulfo-conjugation at lower concentrations (IC_<50>=9.7μg/ml), but only showed weak inhibition toward glucuronosyl conjugation of 1-naphthol in Caco-2 cells. In addition, turmeric was found to strongly inhibit in vitro phenol sulfotransferase (SULT) activity and demonstrate moderate inhibitory properties against UDP-glucuronosyl transferase (UGT) activity in Caco-2 cells (IC_<50>=0.17mg/ml and 0.62mg/ml, respectively). Curcumin also strongly inhibits in vitro phenol sulfotransferase activity with an IC_<50> of 2.4μg/ml. Moreover, and in contrast to the moderate inhibition of UGT activity by turmeric and curcumin, both induce the expression of the UGT1A1 and UGT1A6 genes, revealed by real-time PCR analysis. These findings are indicative of a possible interaction of both turmeric and curcumin with conjugation reactions in the human intestinal tract and colon. This in turn may affect the bioavailability of therapeutic drugs and toxicity levels of environmental chemicals, particularly procarcinogens.
- 2006-07-01
著者
-
田村 悦臣
共立薬科大学
-
Tamura Hiroomi
Kyoritsu University of Pharmacy
-
Saruwatari Ayako
慶応義塾大学 薬学部
-
Tamura Hiroomi
Graduate School Of Pharmaceutical Sciences Keio Univ.
-
Tamura Hiroomi
Graduate School Of Pharmaceutical Sciences Keio University
-
Tamura Hiroomi
Faculty Of Pharmacy Keio University
-
Tamura Hiroomi
Faculty Of Pharmacy Keio University:graduate School Of Pharmaceutical Sciences Keio University
-
OKAMURA Shigeaki
Kyoritsu University of Pharmacy
-
NAGANUMA Megumi
Kyoritsu University of Pharmacy
-
SARUWATARI Ayako
Kyoritsu University of Pharmacy
-
Tamura Hiroomi
Kyoritsu College of Pharmacy
関連論文
- ポリエチレンテレフタレート再生材中のホルムアルデヒド, アセトアルデヒドおよびオリゴマーの分析
- マウス嗅覚器の硫酸転移酵素の活性と発現様式
- ラット肝フェノール硫酸転移酵素の肝臓内局在性
- Triethylamine及び基質による硫酸転移酵素の阻害様式
- ラットhydroxysteroid sulfotransferase ST-20, ST-40の発現制御は異なる
- Effects of Cytochrome P450 Inducers on the Gene Expression of Ocular Xenobiotic Metabolizing Enzymes in Rats
- Changes in the Gene Expression of Drug Metabolizing Enzymes during Cataractogenesis in Shumiya Cataract Rats
- Inhibitory Effects of Various Beverages on the Sulfoconjugation of 17β-Estradiol in Human Colon Carcinoma Caco-2 Cells(Biopharmacy)
- Metabolism of Bisphenol A in the Rat Syncytiotrophoblast Cell Line, TR-TBT 18d-1
- The Effects of Coffee on Conjugation Reactions in Human Colon Carcinoma Cells(Toxicology)
- Coffee Inhibits the Glucuronidation of 1-Naphthol in Rat Syncytiotrophoblast Cells
- Sulfation of Estradiol in Human Epidermal Keratinocyte
- ポリエチレンテレフタレート再生材中の残存金属と再生材使用の判別法
- ポリエチレンテレフタレート再生材中の残存揮発性物質の分析
- Changes in the Expression of Cytochrome P450 Genes in Hemin-Induced Differentiated K562 Cells(Biochemistry)
- Turmeric and Curcumin Modulate the Conjugation of 1-Naphthol in Caco-2 Cells(Miscellaneous)
- Effect of Herbal Teas on Conjugation Reactions in a Human Colon Carcinoma Cell Line, Caco-2
- Retinoic Acids Induce Neurosteroid Biosynthesis in Human Glial GI-1 Cells via the Induction of Steroidogenic Genes
- Expression Profiling of Drug Metabolizing Enzymes in Rat Syncytiotrophoblast Cell Lines
- Coffee Induces Breast Cancer Resistance Protein Expression in Caco-2 Cells
- Sulfoconjugation of Bisphenol A in a Human Neuroblastoma Cell Line, NB-1
- Interferon Gamma Induces Steroid Sulfatase Expression in Human Keratinocytes
- ポリエチレンテレフタレート再生材中の残存揮発性物質の分析
- ポリエチレンテレフタレート再生材中の残存金属と再生材使用の判別法
- Coffee inhibits adipocyte differentiation via inactivation of PPARγ