Essential Role of Mg^<2+> in Flocculation of Saccharomyces diastaticus

概要

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Flocculation of Saccharomyces diastaticus IFO 1958 was studied. Cells of IFO 1958 did not flocculate even in the stationary phase without Mg^<2+> although they began to flocculate strongly 18h after inoculation in the presence of Mg^<2+>. Cycloheximide completely inhibited induction of floe-forming ability of Mg^<2+>-deficient cells. Co-flocculation between flocculent cells and Mg^<2+>-deficient cells was investigated by treatment by proteolytic enzymes and chemical modification. Treatment of Mg^<2+>-deficient cells by proteolytic enzymes did not affect the co-flocculation with flocculent cells. Photo-oxidation or mercaptoethanol-reduction of Mg^<2+>-deficient cells failed to diminish the co-flocculation with flocculent cells while treatment of Mg^<2+>-deficient cells by periodate caused a considerable loss of the co-flocculation. On the other hand, flocculent cells deflocculated by proteolysis or chemical modification of protein component failed to co-flocculate with Mg^<2+>-deficient cells. These findigs suggest that Mg^<2+>-deficient cells are non-flocculent because of lack of protein component essential for flocculation of cells of IFO 1958.
香川大学の論文
2006-03-31