Spectrophotometric Determination of Fluoxetine by Batch and Flow Injection Methods

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A rapid, simple, and accurate spectrophotometric method is presented for the determination of fluoxetine by batch and flow injection analysis methods. The method is based on fluoxetine competitive complexation reaction with phenolphthalein-β-cyclodextrin (PHP-β-CD) inclusion complex. The increase in the absorbance of the solution at 554nm by the addition of fluoxetine was measured. The formation constant for fluoxetin-β-CD was calculated by non-linear least squares fitting. Fluoxetine can be determined in the range 7.0×10^<-6>-2.4×10^<-4> moll^<-1> and 5.0×10^<-5>-1.0×10^<-2> moll^<-1> by batch and flow methods, respectively. The limit of detection and limit of quantification were respectively 4.13×10^<-6> moll^<-1> and 1.38×10^<-5> moll^<-1> for batch and 2.46×10^<-5> moll^<-1> and 8.22×10^<-5> moll^<-1> for flow method. The sampling rate in flow injection analysis method was 80±5 samples h^<-1>. The method was applied to the determination of fluoxetine in pharmaceutical formulations and after addition to human urine samples.
2006-12-01