培養小腸細胞の酸化的ストレスとチオレドキシン

概要

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Hydrogen peroxide caused cytotoxicity of the small intestinal epithelial cell line, IEC-6, judged from an MTT assay and the release of lactate dehydrogenase. The dutathione S-transferase and thioredoxin reductase activities and SH content decreased dose-dependently with H_2O_2, but thioredoxin activity increased at low H_20_2 concentrations. In addition, the increase in thioredoxin activity was tim-dependent during the initial stages of oxidative stress. Reverse-transcription-polymerase chain reaction arrmlification also showed that the mRNA content in IEC-6 cells increased time-dependently at 0.25 mM H_20_2. These results indicate that cellular oxidative shock causes an increase in the activity of thioredoxin, which is involved in the defense mechanism against oxidative stress.
大手前大学・大手前短期大学の論文