Skin Penetration Flux and Lag-Time of Steroids Across Hydrated and Dehydrated Human Skin in Vitro(Biopharmacy)

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概要

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• To study the effect of hydration on skin absorption, we investigated penetration across human skin of twelve model chemicals having steroidal structure but different molecular weight and compared the steady-state penetration rate (J) and lag-time (t) across hydration intact skin (J_h and t_h) with that across dehydrated intact skin (J_d and t_d). Stratum corneum (SC) thickness of hydrated (52μm) is 3.3 times that of dehydrated skin (16μm). Transepidermal water loss (TEWL) of hydrated (7.6±2.1g/m^2/h) is twice that of dehydrated skin (3.4±1.6g/m^2/h, p<0.05) which are similar to in vivo values, suggesting the SC barrier function was recovered. The ratio of J_h/J_d ranged between 0.7 and 3.6 (average of 1.9). On the other hand, the ratio of t_h/t_d was almost constant (average of 0.8). Ratios of J_h/J_d and t_h/t_d were independent of MW and K_<o/w>. In percutaneous absorption experiments in vitro, skin was preserved in culture medium until use and SC might swell during that time. Therefore, we consider the possibility that J and t varied between hydrated and dehydrated skin. We confirmed the difference of J and t between hydrated and dehydrated skin in vitro and now need to define these results under in vivo condition.

• 2006-11-01

著者

• MAIBACH Howard

  Department of Dermatology, School of Medicine, University of California

• Hikima Tomohiro

  Department Of Biochemical Engineering And Science Kyushu Institute Of Technology

• Hikima Tomohiro

  Department Of Bioscience And Bioinformatics Kyushu Institute Of Technology:department Of Dermatology

• Maibach Howard

  Department Of Dermatology University Of California

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