Spectrofluorimetric Determination of Paroxetine Hydrochloride in Its Formulations and Human Plasma

概要

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A sensitive spectrofluorimetric procedure for the determination of paroxetine-HCl in pharmaceutical formulations and human plasma has been described. The native fluorescence of the drug has been studied under different conditions. Maximum fluorescence intensity was obtained in methanol at 340nm using 290nm for excitation. Different surfactants showed negative effect on the fluorescence intensity of paroxetine-HCl. Regression analysis of Beer's plot showed good correlation (r=0.9999) between fluorescence intensity and concentration over the range of 0.05-0.40μgml^<-1> with lower limit of detection (LOD) of 0.015μgml^<-1>. The drug was successfully determined in its tablets with average % recovery of 98.00±0.99% which was in accordance with those given by a compendial method. The method was also applied to the determination of paroxetine-HCl in spiked human plasma with average recovery of 77.70±1.06%.
公益社団法人日本薬学会の論文
2006-04-01