Complex Observation in Electron Microscopy : VI. Comparison of Information Transfer Reliability between Zernike Complex Observation and Transport of Intensity Equation Method(Electromagnetism, optics, acoustics, heat transfer, classical mechanics, and flu
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概要
- 論文の詳細を見る
A transmission electron microscopic (TEM) scheme to reconstitute wavefronts of electrons scattered from objects, which was termed as complex observation (COBS), has been successfully introduced. COBS is based on a complex combination of two orthogonal TEM experiments of the conventional (bright-field) and Zernike phase contrast method, both of which are relying on the interference phenomenon for the phase recovery. Recently, another class of phase recovery, the non-interference method often called as the Transport of Intensity Equation (TIE) method, has been proposed. TIE rather relies on the change of the optical energy or intensity in the direction of wave transfer. We report the methodological comparison between COBS and TIE, which has been carried out with TEM simulations and by using a new index, ITR, which was introduced in a previous paper for the comparison of principal aspects of microscopic schemes. TEM simulation has been clarified the preference of COBS in the image quality. This has been also confirmed quantitatively by using ITR, where the drawback of TIE comes to more visible. TIE inherently requests a differential operation to close the formulation, which is actually problematic for images including noise components. Signals in the low and high frequency regions are both seriously influenced by the noise, which results in a rather low resolution image for objects with a low dose limit such as biological samples.
- 社団法人日本物理学会の論文
- 2006-08-15
著者
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Nagayama Kuniaki
Okazak Institute For Integrative Bioscience National Institutes Of Natural Sciences (nins)
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Nagayama Kuniaki
Okazaki Institute For Integrative Bioscience National Institutes Of Natural Sciences Department Of P
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Sugitani Shouzou
Okazaki Institute For Integrative Bioscience National Institutes Of Natural Sciences Department Of P
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