Analysis of phosphatidylcholine oxidation products in human plasma using quadrupole time-of flight mass spectrometry

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概要

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• We report here an application of the previous method for the analysis ofphosphatidylcholine (PC) and lysophosphatidylcholine (lysoPC) oxidation products inhuman plasma using quadrupole time of flight (Q-TOF) mass spectrometry withelectrospray ionization. We separated these products using an HPLC C8 column witha gradient of methanol and 10 mM aqueous ammonium acetate. Monohydroperoxides,epoxyhydroxy derivatives, oxo derivatives, and trihydroxides of palmitoyl-linoleoyl(C16:0/C18:2) PC and stearoyl-linoleoyl (C18:0/C18:2) PC were detected mainly asMH+ and [M+Na]+ ions in the plasma of alcoholic patients. Using standard syntheticPC-OH (C16:0/C18:2-OH), the lipid extract component was identified as(C16:0/C18:2-OH) PC based on the product ions of ESI-MS-MS. Using standardsynthetic PCOOH (C16:0/C18:2-OOH) as a reference, the PCOOH concentration inplasma was quantified. Two oxidatively modified lysoPCs were also detected. This isthe first report showing the presence of epoxyhydroxy derivatives,monohydroperoxides, oxo derivatives, and trihydroxides of (C16:0/C18:2) PC and(C18:0/C18:2) PC, and PC-OH (C16:0/C18:0-OH) in human plasma.

• 神戸大学の論文

著者

• Yoshioka Naoki

  Kobe University Graduate School Of Medicine

• Adachi Junko

  Kobe University Graduate School Of Medicine

• Ueno Yasuhiro

  Department Of Legal Medicine Kobe University Graduate School Of Medicine

• Ueno Yasuhiro

  Nippon Kokan Koji Co.

• 吉岡 直樹

  慶応大 理工

• Yoshioka Naoki

  Department Of Radiology Institute Of Medical Science University Of Tokyo

• Asano Migiwa

  Kobe University Graduate School of Medicine

• Nushida Hideyuki

  Kobe University Graduate School of Medicine

• Ueno Yasuhiro

  Kobe University Graduate School of Medicine

• Nushida Hideyuki

  Division Of Legal Medicine Department Of Social Medicine Kobe University Graduate School Of Medicine

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