Regulation of α2-Macroglobulin Gene Expression by Interleukin-6 (BSF-2/HSF)
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概要
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In rat hepatocyte primary cultures recombinant human interleukin-6 (rhIL-6) induced α_2-macroglobulin (α_2M) synthesis 54-fold. Half-maximal induction was achieved at a rhIL-6 concentration of 30 pM. RhIL-1β led only to a 2-fold increase in α_2M synthesis, but strongly impaired the action of IL-6. Intraperitoneal injection of rhIL-6 into male rats resulted in a 19.7-fold increase of α_2M mRNA already after 4h. In contrast, α_2M mRNA levels (50-fold increase) were reached between 16 and 24h after intramuscular injection of turpentine. Whereas turpentine-induced inflammation resulted in an increased α_2M synthesis in male and female rats, rhIL-6 injection had no effect in female rats. The increases after rhIL-6 administration in mRNA concentrations were followed by corresponding changes in α_2M levels in serum. By Northern analysis it was demonstrated that LPS-stimulated human monocytes synthesize IL-6 mRNA. The 5'-end of the rat α_2M gene has been isolated and the first 3 exons and 166 base pairs of the 5'-flanking region were identified by a combination of oligonucleotide hybridization and DNA sequencing. The transcriptional start site was determined by RNase protection as well as by primer extension experiments. 5'-CATAAAG-3' and 5'-TCAAAA-3'were found as TATA- and CAAT-box equivalent sequences, respectively. Furthermore, a potential glucocorticoid binding site (5'-TGTTCT-3') was localized on the antisense strand of the α_2M gene.
- 1988-03-17
著者
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Heinrich Peter
Biochemisches Institut Universitat Freiburg
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Heinrich Peter
Biochemisches Institut Aebert-ludwigs-universtat
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ANDUS Tilo
Biochemisches Institut, Universitat Freiburg
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GEIGER Thomas
Biochemisches Institut, Universitat Freiburg
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KLAPPROTH Jan
Biochemisches Institut, Universitat Freiburg
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KUNZ Dieter
Biochemisches Institut, Universitat Freiburg
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HEISIG Michael
Biochemisches Institut, Universitat Freiburg
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CASTELL Jose
Biochemisches Institut, Universitat Freiburg
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Andus Tilo
Biochemisches Institut Universitat Freiburg
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Kunz Dieter
Biochemisches Institut Universitat Freiburg
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Castell Jose
Biochemisches Institut Universitat Freiburg
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Klapproth Jan
Biochemisches Institut Universitat Freiburg
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Geiger Thomas
Biochemisches Institut Universitat Freiburg
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Heisig Michael
Biochemisches Institut Universitat Freiburg
関連論文
- Regulation of α2-Macroglobulin Gene Expression by Interleukin-6 (BSF-2/HSF)
- Professor Katsunuma's Memorial