The Role of the Epstein-Barr Virus(EBV)in Tumorigenesis of EBV(+)Gastric Carcinomas
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概要
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In order to clarify the role of Epstein-Barr virus (EBV) in tumorigenesis in the stomach, 513 gastric carcinoma cases were investigated for the presence of viral UNA, its geno-typing, LMP-1 gene-deletion and EBV monoclonality. Comparison was made with EBVs detected in 7 lymphoma cell lines (LCLs) maintained in severe combined immuno-deficiency (SCID) mice and 18 non-Hodgkin's lymphomas. Thirty-three cases (6.4%) were demonstrated to be positive for EBV by means of EBER-1 RNA in situ hybridization. PCR examination confirmed the presence of EBV-DNA in 32 of these 33 (97%). Clinico-pathologic data showed no statistically significant difference in histologic grading, location of cancer and status of vessel and lymphatic invasion between the EBV positive and negative groups, although the former significantly predominated in the submucosal invasion group. EBV type A was found in 20 of 21 (95%) evaluable cases and type B in 1 (5%). Nineteen (90%) showed a 30bp deletion within the 3' end of LMP-1. EBV monoclonality estimated by tandem repeats of LMP-1 gene was noted in only 12 out of 32 (38%). In comparison, EBVs detected in LCLs in SCID mice and non-Hodgkin's lymphomas were all type A, 7 of 7 and 18 of 18 evaluable cases, respectively, showed a 100% incidence of a specific LMP-1 gene deletion and demonstrated EBV monoclonality in 7 of 7 (100%) and 11 of 19 (58%), respectively. The results indicate that EBVs in gastric carcinoma are rather heterogeneous and indicating that they may play a less important role than cases of LCLs of SCID mice and non-Hodgkin's lymphomas. And, the low incidence of monoclonality suggests that the copy number of EBV may be less in gastric carcinomas.
- 北里大学の論文
- 1997-06-30