Preparation of Tumor Cell Samples of Colorectal and Stomach Cancer : A Preliminary Study for Autologous Vaccination of Active Specific Immunotherapy

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We performed the treatment of tumor cells from surgical specimens to prepare autologous tumor cell samples for active specific immunotherapy. A total of 42 solid tumors were studied. Of these, 20 were primary colon carcinomas, 9 were primary rectum carcinomas, 7 were primary stomach carcinomas and 6 were other tumors including 2 liver metastases from colon carcinomas. The freshly excised tumors were dissociated enzymatically and then cryopreserved in a liquid nitrogen. We investigated the dissociation yield of tumor cells, and the tumor cell viability before and after cryopreservation. The cell dissociation produced a mean yield of 22×10^6 cells/g colon tumor (5-57×10_6 cells/g), 11×10_6 cells/g rectum tumor (3-40×10_6 cells/g) and 16×10_6 cells/g stomach tumor (3-46×10_6 cells/g). The mean prefreeze viability of colon, rectum and stomach tumors was 77% (46〜95%), 77% (67〜93%) and 71% (53〜85%), respectively. There was no significant difference on the dissociation yield and prefreeze viability between those tumors. Two different ways were made on the dilution process to determine the cell viability after cryopreservation ; tumor cells were diluted within 10 min in group A (23 tumors ; 9 colon, 8 rectum and 6 stomach) and with the slower dilution in 60 min in group B (13 tumors ; 11 colon, 1 rectum and 1 stomach). The mean cell viability after thawing was 29% in group A and 74% in group B. The mean cell viability of group B was significantly higher than group A, although there was no significant difference on the mean prefreeze cell viability between both groups. A significant improvement of the cell viability was observed with the slower dilution of tumor cells. We presented here the fundamental aspects of the tumor cell preparation for active specific immunotherapy with autologous tumor cell vaccine for the patients with colorectal and stomach cancer. We could prepare the tumor cell samples with a high viability and cell integrity, which are required for the successful vaccinations. The possibility and problems of applying this procedure into the clinical practice are discussed.
北里大学の論文
1991-02-28

Preparation of Tumor Cell Samples of Colorectal and Stomach Cancer : A Preliminary Study for Autologous Vaccination of Active Specific Immunotherapy

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