ブドウ細胞懸濁培養におけるアミノペプチダーゼ活性の誘導 : アントシアニン生合成と関連して

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キーワード : 二次代謝アントシアニン生合成細胞分裂フェニルアラニンアミノペプチダーゼブドウ懸濁培養　抄録 : Cell suspension culture of Vitis produce anthocyanin after cessation of cell division. Aminopeptidase activities were measured with phenylalaninyl-4-methyl-7-coumarilamide (MCA) and alaninyl-MCA as substrates. Both activities show a peak 3 days after transfer. Hydrolytic activities against phenylalany 1 (Phe)-MCA were increased again 5 day after transfer, while that against alanyl (Ala)-MCA was decreased. When 3 times larger culture was transfer, the time for cessation of cell division and anthocyanin synthesis was advanced by approx. 4 day, the peak of hydrolytic activity against Phe-MCA was also advanced by approx. 4 day. Inhibitor study indicated different enzyme(s) was responsible for hydrolysis of Phe-MCA and Ala-MCA. These results indicated an aminopeptidase(s) that preferentially liberated Phe from peptides was induced in relation to cessation of cell division and/or anthocyanin biosynthesis.
鶴岡工業高等専門学校の論文
2003-12-25