微生物による油脂生産に関する研究 : II 石油炭化水素よりの生産

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Fat production by a hydrocarbon utilizing fat yeast, Candida IF-6A, was studied, which was selected out of 55 strains isolated from 93 samples, including garden soils, oil-soaked soils, sewage water, fruits, etc. Kerosene and glucose were used as the carbon sources. Fat cells containing about 3% cell nitrogen were obtained by reducing the concentration of the nitrogen source in both culture media. The components of the accumulated lipids and the fatty acid composition of the lipids were compared. Cells grown by kerosene contained larger amounts of phospholipid, non-saponifiable matter and odd numbered fatty acids. When the following four n-alkanes, n-dodecane, n-tridecane, n-hexadecane and n-heptadecane, were used as the carbon source, different degree of lipid accumulation and different amount of odd or even numbered fatty acids were found. The degree of fat accumulation was, highest in the case of n-hexadecane, and the amounts of odd numbered fatty acids were much larger in the case of n-tridecane or n-heptadecane than in the case of n-dodecane or n-hexadecane. It is quite obvious that the chain length or the number of carbon of the assimilated hydrocarbon has strong effect upon the fatty acid composition of the accumulated lipids.
明治大学の論文