マウス2,4,6-トリニトロベンゼンスルホン酸(TNBS)誘導性腸炎活動期におけるCD8^+T細胞の機能解析

概要

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We analyzed the functional role of CD8^+T cell receptor (TCR) Vβ14^+T cells which increased specifically in the lamina propria in 2, 4, 6-Trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice. Cytotoxic activity and cytokine production in CD8^+TCR Vβ14^+T cell clones were analyzed by ^<51>Cr release assay and enzyme-linked immunosorbent assay, respectively. Cell transfer studies using these clones were performed. Established T cell clones showed specific cytotoxic activity against TNBS-conjugated self spleen cells, and this cytotoxicity was completely inhibited by anti-TCR Vβ14 monoclonal antibody. These clones produced interferon-gamma (IFN-γ) in their culture supernatant, but neither interleukin (IL) -2 nor IL-4. Histological findings of the colon in mice, which received clones transfer after the enema with suboptimal doses of TNBS, showed massive colitis. Our results indicated that CD8^+TCR Vβ14^+T cells had a cytotoxic T lymphocyte function induced by Thl T cell response and played a pathogenic role in the development of TNBS-induced colitis.
岐阜大学の論文
2003-03-31