Tensile Properties of Contractile and Synthetic Vascular Smooth Muscle Cells(Proceedings of the Switzerland-Japan Workshop on New Directions in Cellular and Tissue Biomechanics)(Special Issue on Bioengineering)

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Tensile properties of vascular smooth muscle cells (VSMCs) of synthetic and contractile phenotypes were determined using a newly developed tensile test system. Synthetic and contractile VSMCs were isolated from the rabbit thoracic aorta with an explant and an enzymatic digestion method, respectively. Each cell floated in Hanks' balanced salt solution of 37℃ was attached to the fine tips of a pair of micropipettes with a cell adhesive and, then, stretched at the rate of 6 μm/sec by moving one of the micropipettes with a linear actuator. Load applied to the cell was measured with a cantilever-type load cell ; its elongation was determined from the distance between the micropipette tips using a video dimension analyzer. The synthetic and contractile VSMCs were not broken even at the tensile force of 2.4 μN and 3.4 μN, respectively. Their stiffness was significantly higher in contractile phenotype (0.17 N/m) than in synthetic one (0.09 N/m). The different tensile properties between synthetic and contractile cells are attributable to the differences in cytoskeletal structures and contractile apparatus.
社団法人日本機械学会の論文
2002-12-15