エポン切片-イオンエッチング-SEM法の骨組織三次元構造解析への応用
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概要
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Bone tissue consists of three cells, namely, osteoblast, osteoclast and osteocyte. Each cell has a role in maintaining the bone tissue. Recent studies suggest that the osteocytes can be mechanosensors, and that it is important to investigate the overall morphology of osteocytes and their network in the bone matrix in detail in order to illuminate the mechanism of forming lamellar structure and remodeling bone tissue according to the external force and gravity. But, since osteocytes are embedded in a mineralized matrix, it is difficult to observe their overall morphology. Therefore the objectives of this study are (1) to establish a method "Epon section-ion etching-SEM" for examining hard tissue, including bone and tooth, (2) to elucidate the overall morphology and arrangement of the osteocyte and its processes in the inner circumferential lamellae and around the Haversian cannal of the cortical bone by three-dimensional reconstruction. The best conditions of ion etching for examining hard tissue were almost the same as those for soft tissues, but it was necessary to change the etching time and etching voltage according to magnifications. From these results, there seem to be some structural differences between the two different areas of a rat's humerus. Around the Haversian cannal, the lamellar structure is not clear, and the arrangement of collagen fibrils and osteocytes are parallel to the axis of the bone. In the area of the inner circumferential lamellae, the lamellar structure is clear, but as for the arrangement of collagen fibrils : some are parallel to, some oblique and some crossing the axis of the bone as well as osteocyte, so both of them are variable. In the inner circumferential lamellae, the osteocyte's processes change and begin to travel on the boundaries of the lamellae. According to our observations, the arrangement of osteocytes is very relevant to that of collagen fibrils.
- 2005-08-25
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