Analysis of Micronuclei in the Transferrin-receptor Positive Reticulocytes from Peripheral Blood of Nasopharyngeal Cancer Patients Undergoing Radiotherapy by a Single-laser Flow Cytometer
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概要
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The automated micronucleus test is now accepted as a simple, objective, and accurate method for evaluating potential mutagenic effects caused by physical, chemical or biotic factors. This paper describes a single-laser flow cytometry, based on an immunomagnetic isolation technique in combination with acridine orange staining, to detect frequencies of micronucleated transferrin-receptor positive reticulocytes from human peripheral blood. Using this flow cytometric system, we detected the frequencies of micronucleated transferrin-receptor positive reticulocytes from 10 nasopharyngeal cancer patients undergoing radiotherapy and the baseline of the frequencies of micronucleated transferrin-receptor positive reticulocytes from 7 healthy donors. The results showed that the mean frequency of micronucleated transferrin-receptor positive reticulocytes from healthy donors was 0.236% and that from nasopharyngeal cancer patients before radiotherapy was 0.297%. After radiotherapy it was significantly elevated. When the cumulative dose of radiotherapy was about 20Gy, it reached a maximum of 6.905%, and then, as the cumulative dose of radiotherapy continued to increase to 30Gy, 40Gy and 50Gy, the frequency decreased to 6.258%, 5.119% and 5.007% respectively. Our results indicated that the single-laser flow cytometric system was quick, reasonable and acceptable for detecting the frequency of micronucleated transferrin-receptor positive reticulocytes from human peripheral blood.
- 日本放射線影響学会の論文
- 2005-03-25
著者
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Liu Jin
Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine
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Liu Jin
Department Of Hygiene Toxicology College Of Preventive Medicine Third Military Medical University
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SUN Li
Department of Hygiene Toxicology, College of preventive Medicine, Third Military Medical University
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LI De
Radiation Therapy Treatment Center, Xinqiao Hospital, Third Military Medical University
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LIU Zhi
Radiation Therapy Treatment Center, Xinqiao Hospital, Third Military Medical University
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YANG Lu
Department of Hygiene Toxicology, College of Preventive Medicine, Third Military Medical University
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CAO Jia
Department of Hygiene Toxicology, College of Preventive Medicine, Third Military Medical University
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Li De
Radiation Therapy Treatment Center Xinqiao Hospital Third Military Medical University
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Yang Lu
Department Of Hygiene Toxicology College Of Preventive Medicine Third Military Medical University
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Cao Jia
Department Of Hygiene Toxicology College Of Preventive Medicine Third Military Medical University
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Sun Li
Department Of Hygiene Toxicology College Of Preventive Medicine Third Military Medical University
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Sun Li
Department Of Applied Physics And Materials Research Center The Hong Kong Polytechnic University
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Liu Jin
Department Of Dermatology University Hospital Of Zurich
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Liu Zhi
Radiation Therapy Treatment Center Xinqiao Hospital Third Military Medical University
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YANG Lu
Department of Electrical and Computer Engineering, UC Davis, USA
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LIU Jin
Department of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University
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