Genomic Diversity of Bradyrhizobium Strains Compatible with Soybean (Glycine max L. Merr.) Harboring Various Rj-genes

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概要

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• Based on the different sequence of putative Rj-gsn gene reserved in Brandyrhizobium strains, which was concerned with the compatibility between some Rj-genotype soybeans and some Brandyrhizobium strains, the classification depended on sequence of genomic DNA of Brandyrhizobium strains of each nodulation type was thought possible to analyze by the method of restriction fragment length polymorphism(RFLP). Different rate-cutting enzymes Ase Ⅰ, DraⅠ,SpeⅠ and XbaⅠ were chosen in order to ideally produce fragments greater than 200kbp. The dendrogram of Ase Ⅰwas shown that type C strains were found mainly in one of two clusters, and type B strains in the other cluster. The type A strains were found in both clusters at similar frequency. The dendrogram by Dra I was spilt into three clusters, and the type B and C strains were found exclusively in different cluster. The third cluster had all three types almost uniformly. That of Sph I formed small cluster containing type B strains, but it was recognized no tendency that ach nodulation type strains formed cluster. That of Xba I was recognized no tendency that each nodulation type strains in compared with that of Dra I. The results of cross-agguligation reaction between each nodulation type strains with the antiseru, of those typical A and B in this experiment. Also, weak cross-reaction was observed between some strains of type A and C (Ishizuka et al. 1991). These experimental results indicated that the type B strains were distantly related to the type C strains in regard to the structure of genomic DNA and that of surface of rhizobal cell, the type A strains were located intermediate between type B and C.

• 九州大学の論文

著者

• Eriguchi Masaharu

  Nagasaki Agricultural & Forestry Experiment Station

• Yamakawa Takeo

  Faculty Of Agriculture Kyushu University

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