低カルシウム食飼育ウサギの腹腔内に埋入した骨粒子に集まる細胞についての基礎的研究

概要

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To obtain osteoclasts, bovine bone particles were implanted intraperitoneally into low calcium-diet rabbits. The cells gathered around bone particles were induced to osteoclasts by culture. The most effective method to obtain precursors was, to rabbits breed on a low calcium-diet for 4 weeks, and then implant bone particles intraperitoneally for 3 weeks. When the cells harvested from the rabbits were cultured in a medium containing 10% serum obtained from a low calcium-diet rabbit, several TRAP-positive cells were observed after a 7-day culture. When normal-diet rabbit serum or fetal bovine serum was used, few TRAP-positive cells were observed. These findings suggest that the serum obtained from low calcium-diet rabbits contains some factors for differentiation of cells to TRAP-positive cells. The formation of resorption lacunae on the bone surface and the influence calcitonin suggested that the TRAP-positive cells were osteoclasts. In conclusion, the low calcium-diet rabbits used in this experiment were useful for harvest of precursors of osteoclasts, and the serum from low calcium-diet rabbits is effective to induce and differentiate osteoclasts in vitro.
福岡歯科大学学会の論文
1997-09-30