生活歯髄切断後の覆髄剤としての粉末サンゴの組織反応に関する病理組織学的研究

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As natural powdered corals appear to be effective as dental pulp capping materials, they were analyzed with x-ray detection energy dispersive analysis (Kevex 7000Q) and with x-ray diffraction (experiment 1). The effects of the natural powdered coral for use as dental pulp capping material, on the surrounding tissues implanted (experiment 2) and on the dog dental pulp amputated (experiment 3) were elucidated histo-pathologically and ultrastructurally at various intervals after treatment. For experiment 2,two types of natural corals powdered namely, white and porous, A, and red and dense, B, and the two heated at 700℃ for 30 min, which were previously kept in the polyethylene tubes (3.0×7.0mm), were implanted subcutaneously into the dorsal tissues of 36 rats for 2 to 6 months. For experiment 3,natural powdered coral A and A heated were placed on the dog dental pulp of 64 teeth, coronal pulp of which was previously amputated with round bars (#5) and excavators sterilized, and treated rountinely for 1 to 7 weeks after treatment. As a control, calcium hydroxide was placed on the dental pulp of 12 dog teeth instead of the natural powdered coral for the same intervals. At various intervals after treatment for both experiments, the surrounding tissue was carefully removed, fixed, embedded in paraffin or E pon 812 and sectioned routinely for light-and scanning or transmission electron-microscopy. The degree of the inflammatory reaction in the surrounding connective tissues to 4 types of corals implanted subcutaneously was slightly different each other. In particular, the tissues adjacent to natural coral A were more strongly encapsulated with the dense fibrous tissues than those adjacent to the other coral at all intervals after treatment. However, in the tissues adjacent to the concaved surfaces of all types of coral implanted, the inflammatory response remained at all intervals. The dentin bridge was not positively formed over the dental pulp amputated at 1 to 3 weeks after natural or heated coral A treatment, whereas at 5 to 7 weeks the bonelike dentin bridge was observed on 50% of the experimental teeth treated with natural coral A, but not with heated coral A. The necrosis of the dental pulp adjacent to natural and heated coral A were not observed over the experimental periods. In the heated coral A and B, and natural coral A and B, Ca, P, Mg, Fe and Al ions were observed but the ratio of Mg and Al was different in the A and B types of corals. Natural coral A showed a characteristic peak as observed with calcite.
福岡歯科大学学会の論文
1992-06-30

生活歯髄切断後の覆髄剤としての粉末サンゴの組織反応に関する病理組織学的研究

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